{"id":83,"date":"2026-10-27T21:36:19","date_gmt":"2026-10-28T02:36:19","guid":{"rendered":"https:\/\/sites.austincc.edu\/biology\/lab-manuals-study-guide\/biol-1406-cellular-and-molecular-biology-lab-manuel-12-ed\/"},"modified":"2026-10-27T21:36:19","modified_gmt":"2026-10-28T02:36:19","slug":"biol-1406-cellular-and-molecular-biology-lab-manuel-12-ed","status":"publish","type":"page","link":"https:\/\/sem.austincc.edu\/biology-dev\/lab-manuals-study-guide\/biol-1406-cellular-and-molecular-biology-lab-manuel-12-ed\/","title":{"rendered":"BIOL 1406 \u2013 Biology for Science Majors I Lab Manual, 12 ed."},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><strong>Written by:<br>\nBiology 1406 Faculty<br>\nAustin Community College<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Contributing writers:<\/strong><br>\n<strong> Salvatore Tavormina, Patricia Phelps, David Froehlich, Jacquelyn Jarzem, and Linnea Fletcher<\/strong><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\" \/>\n\n\n\n<ul class=\"wp-block-list acc-resource-list\">\n<li><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVX0k4THNiOFFwTHc\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Biol 1406 Lab Manual Title Page<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVT0ZncUNiQlh4TFk\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Biol 1406 Lab Manual Table of Contents<br><br><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVNENwX0ZVdy1YZGc\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Biol 1406 Lab Introduction<br><br><\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVRmJYeU1wUUdGLUE\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Biol 1406 Safety Worksheet<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVZGdzQmExcjhOZlU\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Exercise 1\u2013How do scientists collect and analyze data?<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVRlZIN05FUmJjOTg\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Exercise 2\u2013How do scientists prepare solutions with specific concentrations of solutes?<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVNHlmX3FTbU1CV0k\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Exercise 3\u2013A. Understanding pH and buffers; B. Introduction to the brightfield microscope<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVVVpIVDZTYm80Wjg\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Exercise 4\u2013Cell structure and diversity<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVSF9yVndqLUpqQ0k\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Exercise 5\u2013How are substances transported within cells and across cell membranes?<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVM2cxUHJnalhtMHc\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Excercise 6\u2013What factors affect enzyme activity?<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVb0w2Q2duWkV2Sjg\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Exercise 7\u2013How is thin layer chromatography used to separate a mixture of molecules?<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVRW1IMXVBZUVUd0k\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Exercise 8-1\u2013How can \u03b1-lactalbumin be separated from the other molecules in milk?<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVeE9rQl81OHZWV0U\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Exercise 8-2\u2013What is the concentration of protein in your milk fractions?<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVOWtqZTFiNFoyYnc\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Exercise 8-3\u2013How can gel electrophoresis be used to separate the proteins present in your milk fractions?<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVZ1NaWTcxaGJFVjg\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Exercise 8-4\u2013How can gel electrophoresis be used to analyze the milk fractions that were collected during your purification of \u03b1-lactalbumin?<\/a><\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVejVYQlhKTld1NjA\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Exercise 9-1\u2013Transformation of E. coli and digestion of plasmid DNA with restriction enzymes<\/a><br>\n<a href=\"http:\/\/www.austincc.edu\/biology\/labmanuals\/140612th\/12th1406lab09B.pdf\"><br>\n<\/a><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVRER6VFJxbVMzOEk\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Exercise 9-2\u2013Which unknown plasmid sample had no plasmids, which had non-recombinant pUC18 plasmids, and which had recombinant pUC18 plasmids?<\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVV0kyVkpDUDR2b1U\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Exercise 10\u2013Using Biological Databases on the Internet<\/a><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td>\n<a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVUmFYMThvNHhkZjQ\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Appendix A<\/a>\n<\/td><td>\n<a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVS1JKTUFVX3JtOWc\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Appendix B<\/a>\n<\/td><td>\n<a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVUHUtaGV2ZndBVU0\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Appendix C<\/a>\n<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td>\n<a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVMzVvcWJjYWxUNlE\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Appendix D<\/a>\n<\/td><td>\n<a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVWTI2eXdRYmJQOGc\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Appendix E<\/a>\n<\/td><td>\n<a href=\"https:\/\/drive.google.com\/file\/d\/0BxZ7UnOPp6OVOFZ2MEJlYXBibGM\/view?usp=sharing\" target=\"_blank\" rel=\"noreferrer noopener\">Appendix F<\/a>\n<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">\n<strong>ISBN: BIOL140612<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><br>\n\u00a9 2006 Austin Community College, Austin Texas<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Written by: Biology 1406 Faculty Austin Community College Contributing writers: Salvatore Tavormina, Patricia Phelps, David Froehlich, Jacquelyn Jarzem, and Linnea Fletcher Exercise 9-1\u2013Transformation of E. coli and digestion of plasmid DNA with restriction enzymes Exercise 9-2\u2013Which unknown plasmid sample had no plasmids, which had non-recombinant pUC18 plasmids, and which had recombinant pUC18 plasmids? Exercise 10\u2013Using [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":62,"menu_order":58,"comment_status":"closed","ping_status":"closed","template":"page-department","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-83","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/sem.austincc.edu\/biology-dev\/wp-json\/wp\/v2\/pages\/83","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sem.austincc.edu\/biology-dev\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sem.austincc.edu\/biology-dev\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sem.austincc.edu\/biology-dev\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/sem.austincc.edu\/biology-dev\/wp-json\/wp\/v2\/comments?post=83"}],"version-history":[{"count":0,"href":"https:\/\/sem.austincc.edu\/biology-dev\/wp-json\/wp\/v2\/pages\/83\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/sem.austincc.edu\/biology-dev\/wp-json\/wp\/v2\/pages\/62"}],"wp:attachment":[{"href":"https:\/\/sem.austincc.edu\/biology-dev\/wp-json\/wp\/v2\/media?parent=83"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}